Calcium channels are vital to neural signaling, specifically in the release of neurotransmitters from axon terminals. In invertebrates, N-type calcium channels assist in neural signaling. Invertebrate calcium-activated chloride channels affect ion transport across cell membranes and help regulate cell homeostasis as well as neural signaling capacity (Wang et al. 2013). Aeolosoma is a freshwater annelid capable of complete regeneration when bisected. We tested whether an N-type Calcium Channel inhibitor and a calcium-activated chloride channel inhibitor would have on cell proliferation and neural regeneration. We found that both inhibitors limited the success of regeneration and had a variety of non-normal effects of neural regeneration.